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- Submitted by: assad111
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- Category: English: Authors
- Date Submitted: 06/11/2013 04:38 AM
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Results
Results
Method (Figure 1)
* Stage 1 (Invadopodia Formation)
Invasive cells are plated on a thick layer of matrigel, about tens to hundred of microns thick
* Stage 2 (Invadopodia Elongation)
Invadopodia elongate before the invasive cell penetrates the underlying tissue; where cells are not chemoattracted and made to migrate in the x-y plane
* Stage 3 (Cell Entrance into the Stromal Compartment)
Cells degrade a thin matrigel layer before passing through the empty pores of the filter
Method (Figure 1)
* Stage 1 (Invadopodia Formation)
Invasive cells are plated on a thick layer of matrigel, about tens to hundred of microns thick
* Stage 2 (Invadopodia Elongation)
Invadopodia elongate before the invasive cell penetrates the underlying tissue; where cells are not chemoattracted and made to migrate in the x-y plane
* Stage 3 (Cell Entrance into the Stromal Compartment)
Cells degrade a thin matrigel layer before passing through the empty pores of the filter
Abstract
The basement membrane (BM) acts as a physiological barrier to maintain tissue homeostasis. Studies (e.g. Cancer Cell Invasion) have shown that BM transmigration is a conserved three-stage process. Firstly, invadopodia-like protrusions form at the ventral surace of invasive cells; later one protrrusion elongates that lastly drives the infiltration of cells into the underlying compartment.
Abstract
The basement membrane (BM) acts as a physiological barrier to maintain tissue homeostasis. Studies (e.g. Cancer Cell Invasion) have shown that BM transmigration is a conserved three-stage process. Firstly, invadopodia-like protrusions form at the ventral surace of invasive cells; later one protrrusion elongates that lastly drives the infiltration of cells into the underlying compartment.
Stage 1
Cancer cells degrade the matrix by producing dark spots in the fluorescent background. Invadopodia are then defined as actin puncta that localize to sites of matrix...
Results
Method (Figure 1)
* Stage 1 (Invadopodia Formation)
Invasive cells are plated on a thick layer of matrigel, about tens to hundred of microns thick
* Stage 2 (Invadopodia Elongation)
Invadopodia elongate before the invasive cell penetrates the underlying tissue; where cells are not chemoattracted and made to migrate in the x-y plane
* Stage 3 (Cell Entrance into the Stromal Compartment)
Cells degrade a thin matrigel layer before passing through the empty pores of the filter
Method (Figure 1)
* Stage 1 (Invadopodia Formation)
Invasive cells are plated on a thick layer of matrigel, about tens to hundred of microns thick
* Stage 2 (Invadopodia Elongation)
Invadopodia elongate before the invasive cell penetrates the underlying tissue; where cells are not chemoattracted and made to migrate in the x-y plane
* Stage 3 (Cell Entrance into the Stromal Compartment)
Cells degrade a thin matrigel layer before passing through the empty pores of the filter
Abstract
The basement membrane (BM) acts as a physiological barrier to maintain tissue homeostasis. Studies (e.g. Cancer Cell Invasion) have shown that BM transmigration is a conserved three-stage process. Firstly, invadopodia-like protrusions form at the ventral surace of invasive cells; later one protrrusion elongates that lastly drives the infiltration of cells into the underlying compartment.
Abstract
The basement membrane (BM) acts as a physiological barrier to maintain tissue homeostasis. Studies (e.g. Cancer Cell Invasion) have shown that BM transmigration is a conserved three-stage process. Firstly, invadopodia-like protrusions form at the ventral surace of invasive cells; later one protrrusion elongates that lastly drives the infiltration of cells into the underlying compartment.
Stage 1
Cancer cells degrade the matrix by producing dark spots in the fluorescent background. Invadopodia are then defined as actin puncta that localize to sites of matrix...